Die Gedenkstätte hatte neue Gutachten in Auftrag gegeben, nachdem geschichtsrevisionistische Kreise die Verbrechen der SS und der Echtheit der überlieferten menschlichen Überreste immer wieder in Frage gestellt hatten.
Read MoreDie Tropen-Lösung
DNA-Labor in Vietnam
Read MoreForensic DNA Samples: Collection and Handling
Chapter in Molecular Analyses
Read MoreDNA typing in forensic medicine and in criminal investigations: a current survey
A brief overview is given of past and present DNA typing and the establishment of forensic DNA databases in Europe.
Read MoreA routine rape case that became tricky (and educational)
After attending this presentation, attendees will understand why it is important to listen to the victim and relatives, who do not fully understand the objective DNA evidence, but who point in a relevant direction relating to procedural failures of police and crime labs.
Read More(GTG)5 allows the distinction between different isolates of the nematode Caenorhabditis elegans
We have not been able to distinguish different isolates from the nematode Caenorhabditis elegans by morphological means. However, they differ on the molecular level and strains from several geographic regions can be identified with the help of "genetic fingerprints" using the oligonucleotide probe (GTG)5.
Read MoreAllele frequency distributions of the polymorphic STR loci HUMVWA, HUMFES, HUMF13A01 and the VNTR D1S80 in a Filipino population from Metro Manila
Allele frequency distributions at the short tandem repeat (STR) loci HUMVWA, HUMFES, HUMF13A01 and of the variable number of tandem repeat (VNTR) locus D1S80 were determined in a Filipino population from Metro Manila (103 individuals) by use of the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE).
Read MoreShort tandem repeat (STR) locus HUMD8S306 in a large population sample from Germany
Applied DNA typing in medico-legal investigations, in criminalistic practice, and in paternity cases often relies on high inclusion and exclusion probabilities.
Read MoreInterdisziplinäres Fachforum: Der Fall Mirco
Das Bremer Fachforum stellt mit dem Mord an Mirco in NRW im Jahr 2010 dar, wie ein sehr schwieriger Fall technisch, organisatorisch und gedanklich bearbeitet und gelöst werden konnte.
Read MoreThe quantitative alteration of the DNA content in strangulation marks is an artefact
It is frequently difficult to prove the vitality in cases of fatal hanging. Statistical analysis demonstrated a significant correlation between elevated DNA content and reduced water content of strangulation marks.
Read MoreY-chromosomal short tandem repeat haplotypes at the loci DYS393, DYS19, DYS392, and DYS385-I/II, DYS390, DYS389-I/II, and DYS391 in a Filipino population sample
Source: J Forensic Sci (2001) 46(5): 1250–3.
Miranda JJ, Benecke M, Hidding M, Schmitt C
The article as .pdf
Population: Male population sample (n = 106) from the Metro Manila area (largest urban center in the Philippines).
Keywords: forensic science, forensic DNA typing, short tandem repeat (STR), Y chromosome, genetic fingerprint, Philippines
Whole blood samples were obtained from 106 unrelated male individuals living in Metro Manila, Philippines, through the Department of Health, Manila. DNA was extracted by isopropanol fractionation-sodium iodide precipitation (4) and quantified by spectrophotometry. Nine Y-chromosomal short tandem repeats (STR’s) were analyzed from a population sample of 106 unrelated males by means of a quadruplex PCR (DYS393, DYS19, DYS392, DYS385-I/II) and a triplex PCR (DYS390, DYS389-I/II, and DYS391).
Primers were Cy5-labeled, and based on sequences described by Kayser et al. (1). PCR products were separated on ReproGel™ High Resolution polyacrylamide gels, and laser-detected by an ALFexpress sequencer (Amersham Pharmacia Biotech). Allelic ladders, and nomenclature were standardized against allelic ladders from P. de Knijff (Leiden), L. Roewer (Berlin), J. Edelmann (Leipzig), and P. Schneider (Mainz).
Discrimination capacity for the nine-loci system was 83%. Gene diversity was calculated following Kayser et al. (3). Frequencies of the individual alleles are shown in Table 2. Haplotype data (88 distinct haplotypes, 75 of which were unique) are given in Table 1. Gene diversity values ranged between 0.37 for DYS91 and 0.94 for DYS385, which is similar to frequences reported elsewhere (2,3).
Acknowledgments
The authors wish to acknowledge the assistance of Rubigilda Paraguison and Edith Tria in the collection and preparation of the samples. Dr. Miranda was supported by a fellowship of the Deutscher Akademischer Austauschdienst (DAAD, German Academic Exchange Service), Federal Republic of Germany.
References
1. Kayser M, Caglia A, Corach D, Fretwell N, Gehrig C, Graziosi G, et al. Evaluation of the Y-chromosomal STRs: a multicenter study. Int J Legal Med 1997;110:125–33.
2. Pestoni C, Cal ML, Lareu MV, Rodriguez-Calvo, Carracedo A. Y chromosome STR haplotypes: genetic and sequencing data of the Galician population (NW Spain). Int J Legal Med 1998;112:15–21.
3. Rossi E, Rolf B, Schürenkamp M, Brinkmann B. Y-chromosome STR haplotypes in an Italian population sample. Int J Legal Med 1998;112: 78–81.
4. Wang L, Hirayasu K, Ishizawa M, Kobayashi Y. Purification of genomic DNA from human whole blood by isopropanol fractionation with concentrated NaI and SDS. Nucleic Acids Res 1994;22:1774 –5.
A homicide in the Ukraine
In an apartment, bone fragments were found in a fireplace. Furthermore, a varnished skull was found elsewhere in the same apartment.
Read MoreCoding or non-coding, that is the question
Having solved the last technical hurdles to extract DNA information from virtually any biological material, forensic biologists now have to ponder the ethical and social questions of using information from exonic DNA.
Read MoreAsian online Y-STR Haplotype Reference Database
For several years Y-chromosomal microsatellites (short tandem repeats, STRs) have been well established in forensic practice. In this context, the genetic characteristics of the Y chromosome (i.e. its paternal inheritance and lack of recombination) render STRs particularly powerful.
Read MoreDXS10011: studies on structure, allele distribution in three populations and genetic linkage to further q-telomeric chromosome X markers
The hypervariable tetranucleotide STR polymorphism DXS10011 is a powerful marker for forensic purposes.
Read MoreRandom amplified polymorphic DNA (RAPD) typing of necrophageous insects (diptera, coleoptera) in criminal forensic studies: validation and use in practice
To permit quick identification of arthropods, random amplified polymorphic DNA typing (RAPD) was used to support classical morphological and medico-legal analysis of maggots on a human corpse.
Read MoreSexual Assault and Murder: When DNA Does Not Help Even Though It Is Present
Two cases demonstrate that full reliance on biological stains may lead to either wrongful convictions or no prosecution at all.
Read MoreSERO NEWS (DIGITAL) 1996—2006
Digitalisat der Zeitschrift "SeroNews". Mit Herzblut erstellt von Wolfgang Huckenbeck (Inst. f. Rechtsmedizin der Heinrich-Heine-Universität Düsseldorf) & Team.
Read MoreAll Mark Benecke Publications
Veröffentlichungen von MB
Read MoreWir lassen die Spuren sprechen
Warum Wissen über Brechbohnen bei der Aufklärung helfen kann und was Spider-Man mit seiner Karriere zu tun hat, darüber hat der Kriminalbiologe Mark Benecke mit Anja Goerz gesprochen.
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